Polymerase Chain Reaction (PCR).
A DNA
sample can be copied many times (amplified) in vitro in a 3-step cycle.
- Denaturation:
Heating
the DNA
to separate the double-stranded molecule into single strands.
- Annealing:
Cool the mixture to allow complementary primers
to hydrogen-bond to the single strands.
- Extension:
Add DNA polymerase and nucleotides to replicate each strand.
continue
Review: